FEEDSTOCK/PRODUCT CHARACTERIZATION
BIOPROCESSES: ENZYMATIC HYDROLYSIS & FERMENTATION
SYNTHETIC BIOLOGY AND METABOLIC ENGINEERING
- Liquid Chromatograph HPLC
- Carbohydrates/sugars
- Organic acids
- Inhibitors
- HP-SEC average molecular weight
- Gas Chromatograph GC-FID & MS
- Methanol, ethanol, butanol
- Lipids
- Elemental Analysis
- Proteins
- Process parameters optimization in:
- From microplates: < 1ml
- Erlenmeyers or flasks: up to 1L
- Bioreactors: 2-5L
- Batch, Feed-batch, continuous
- Adjustable agitation speed, air/gas ratios, pH, nutrients
- Gas-fermenter (ATEX): 10L (propietary design)
- Laminar air flow cabinet & Anaerobic cabinet
- Incubators with adjustable agitation, temperature, gas inlet
- Online analysis for sugars and nutrients
- Online analysis of exhaustive O2 and CO2 gases
- Genetic tools available for aerobes and anaerobes (pMTLN0000 set, pBAD, pK18mob) pathway assembly, gene expression systems, protein purification, enzyme characterisation.
- Multibioreactor for adaptive evolution
- In-house DNA sequencer (Oxford Nanopore technologies), thermal cycler, spectrophotometer, electrophoresis system, gel imaging system, automation system (Tecan M Nano+)
- Microscope
- Molecular Biology specialized software (SnapGene).
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