CHARACTERIZATION LABORATORY
FEEDSTOCK, BIOMASS AND PELETS CHARACTERIZATION




Determination |
Method |
Sample Conditioning | Stabilization and grinding |
Determination of cellulose, hemicellulose, lignin (acid-insoluble + acid-soluble), and acid-insoluble ash | LAP 002 NREL (“Determination of structural Carbohydrates and lignin in Biomass”) |
Dry Weight | UNE-EN 14774-2:2009 |
Proteins | UNE-EN 15104:2011. Elemental analysis (factor 6.25) |
Ash | UNE-EN 14775:2010 |
Determination of insoluble solids content (WIS: water-insoluble solids) | NREL-LAP: “Determination of Insoluble Solids in Pretreated Biomass Material” |
Determination of soluble oligomer content in the liquid fraction | NREL-LAP “Determination of Sugars, Byproducts, and Degradation Products in Liquid Fraction Process Samples.” |
Determination of inhibitors content | NREL-LAP “Determination of Sugars, Byproducts, and Degradation Products in Liquid Fraction Process Samples.” |
Determination of Extractives | NREL (“Determination of Extractives in Biomass”) |
Starch Determination | Enzymatic method |
Xylose Determination | AOAC Total Starch – Enzymatic kit |
Proteins (acid digestion) | Recommended protocol REGULATION (EC) no152/2009 |
Total Lipids | Folch Method |
Fatty acid methyl ester (FAME) | NREL-LAP “Determination of total lipids as fatty acid methyl esters (FAME) by in situ transesterification” |
HPLC Chromatography Analysis | Glucose, Xyl-Fru-Gal, arabinose, cellobiose, maltose, maltotriose
Acetic acid, lactic acid, succinic acid, levulinic acid, and glycerol Organic acids (C2-C10): Acetic Acid, Propionic Acid, Butyric Acid, Valeric Acid, Hexanoic Acid, Heptanoic Acid, Octanoic Acid, Nonanoic Acid, Decanoic Acid |
GC-FID Chromatography Analysis | Ethanol, methanol, butanol, acetone
Polyhydroxyalkanoates (PHAs) |
TEST |
METHOD |
Moisture content – as received (AR) | UNE-EN ISO 18134-2:207 |
Particle size distribution, dp > 3.15mm | UNE-EN ISO 17827-1:2016 |
Bulk density (“bulk”) | UNE-EN ISO 17828:2016 |
Particle size distribution, dp < 3.15mm | UNE-EN ISO 17827-2:2016 |
Fine particle content in pellet samples | UNE-EN ISO 18846:2017 |
Mechanical durability of pellets | UNE-EN ISO 17831-1:2016 |
Ash content | UNE-EN ISO 18122:2023 |
Unburnt content | Internal Method |
Volatile content | UNE-EN ISO 18123:2024 |
Gross calorific value (GCV) | UNE-EN ISO 18125:2018 |
Elemental analysis (CHN) | UNE-EN ISO 16948:2015 |
GCV + NCV | UNE-EN ISO 18125:2018 |
Sulfur content | UNE-EN ISO 16948:2015 |
Chlorine content | UNE-EN ISO 16994:2017 |
Major elements content (Al Ca Fe Mg P K Si Na Ti) | UNE-EN ISO 16994:2017 |
Minor elements content (As Cd Co Cr Cu Hg Mn Mo Ni Pb Sb V Zn) | UNE-EN ISO 16967:2015 |
Ash fusibility | UNE-EN ISO 16968:2015 |
Determination of Na and K content | UNE-EN ISO 21404:2021 |