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  • Since 2013
  • 1,5t biomass/week

PRETREATMENT

SOLID / LIQUID FRACTIONATION

Biological Processes (Enzymatic Hydrolysis & fermentation)

LABORATORY:

Feedstock / Product characterization

  • Liquid Chromatograph HPLC
    • Carbohydrates/sugars
    • Organic acids
    • Inhibitors
    • HP-SEC  average molecular weight
  • Gas Chromatograph GC-FID & MS
    • Methanol, ethanol, butanol
    • Lipids
  • Elemental Analysis
    • Proteins

Fractionation /
Separation

  • 4 pressurized stirred tank reactors (from 50 ml to 4L) :
    • Up to 220 bar
    • Up to 450ºC
    • Pressure control
    • Gas injection possibility
  • Filtration system: MF, UF & NF
    • Up to 15 bar
    • Permeate flow: 0,03-6 L/h
    • Interchangeable membrane module

Enzimatic Hydrolisis & Fermentation

  • Process parameters optimization in:
    • From microplates: < 1ml
    • Erlenmeyers  or flasks: up to 1L
    • Bioreactors: 2-5L
      • Batch, Feed-batch, continuous
      • Adjustable agitation speed, air/gas ratios, pH, nutrients
  • Chambers for aerobic/ anaerobe microorganisms
  • Incubator with adjustable agitation, temperature, gas inlet

PILOT PLANT:

Pretreatment

  • Continuous horizontal reactor
  • Feed flow: up to 5 kg/h
  • Pressure up to 14.5 bar
  • Temperature up to 200ºC
  • High flexibility in feedstocks

Enzymatic Hydrolysis

  • High solids enzymatic hydrolysis stirred tank reactor
  • 200l capacity
  • Temperature: 20-80 ºC
  • Atmospheric pressure

Fermentation

  • Fully monitored bioreactors
  • 40l and 100l capacity
  • Temperature: 23-80ºC
  • Adjustable agitation speed, air/gas ratios, pH, nutrients

INDUSTRIAL PLANT:

Modular and flexible facility. Main equipments:

  • High solid enzymatic reactors (2 x 3 m3)
  • Press Filter/Centrifuge for solid- liquid separation,
  • Detoxification  of inhibitors
  • Liquid fraction concentration
  • Fermentation train consisting of several monitorized bioreactors (1, 3 and 6 m³)