Upscaling of a fermentation process for recombinant protein production

Scaling up of a fermentation process  for the production of a recombinant protein with a methylotrophic yeast:

• Streams adaptation
• Fed-batch fermentation
• Culture centrifugation
• Biomass storage and delivery

• Adaptation of the process to the pilot plant and inoculum train preparation.
• Fermentation in 100 L bioreactor, including analytics to determine the evolution of the process.
• Data recording of relevant process parameters.
• Separation of the supernatant for pellet recovery
• Delivery to customer